Pci neo vektor

We diluted the vectors pMuLE ENTR CMV L1-R5, pMuLE ENTR CMV L5-L2, and pDEST-NEO to 150 ng/uL as described in the kit's protocol, and used 1ul of each vector and 5 ul of TE buffer, 2ul enzyme RT

FEBS Lett., 587 (22): 3656-3660, 2013. Expression was confirmed by the depositor with western blotting. pCI-neo Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

22.03.2021 Pci neo vektor

Use pCI neo+ vector for fast, easy, and consistent DNA/RNA Purification, Antibody/Protein Purification, Cell Isolation. Tel: 1-631-626-9181 (USA) 44-208-144-6005 (Europe) Email: 2019-nCoV Research Solution Guide; Exact Match The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. Full sequence for pCI-neo shared on Benchling. Mammalian cell expression vector with the CMV promoter and a neomycin-resistance marker. Benchling failed to load. Try refreshing the page.

pCI-neo vector backbone. + Sequence information. + Datasheet. + Compare & Order pCI-neo vector backbone products + TOP customer support.

pCI-neo vector backbone. + Sequence information. + Datasheet. + Compare & Order pCI-neo vector backbone products + TOP customer support.

The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G418.

pCI-neo Mammalian Expression Vector GenBank® Accession Number U47120 and vector sequence text file. Transfection and selection data related to pCI-neo in cell-culture. The K7M2-pCI Neo cell line was derived from the K7M2 wt (ATCC CRL-2836) cell line.

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pCI-neo empty vector and Cx43/pCI-neo plasmid DNA was purified using the EndoFree Plasmid Mega Kit (Qiagen, Germantown, MD, USA International Reagent Resources (IRR) was established by the Centers of Disease Control (CDC). The CDC-IRR is a biological reagent repository established to provide better access to influenza virus strains and research reagents. In this study, a plasmid pCI-J7 was constructed by inserting the entire coding region of PAI-2 into the EcoRl site of the pCI-neo expression vector. The same vector was also used for the construction and expression of an inactive PAI-2, which contained an alanine for arginine substitution at the PI residue at position 380 (pCI-J7ili3go). following groups: Control (untreated), PCI-neo (vector control), 5/6 nephrectomy, and PCI-neo-HGF. Rats were sacrificed at both the fifth and ninth week after 5/6 nephrectomy.

pCI-neo Mammalian Expression Vector Similar to pCI Mammalian Expression Vector Also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells Can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418 pCI-neo vector backbone. + Sequence information. + Datasheet. + Compare & Order pCI-neo vector backbone products + TOP customer support. pCI-neo 5.5kb A m p R C S i n t r o p B R 2 2 o r i Z e o c i n S V 4 0 p r o m o t e r N e o R f 1 o r i.

pcDDNA4/HisMax) 製品概説（特長、他社発現ベクター[vs.pcDNA3/pBK-CMV ]とのトランジェントおよびステイブルトランスフェクションにおける発現比較） Article Snippet: The cDNA coding for FGFR2c was amplified by pMD18T-simple FGFR2c (HG10824-M, Sino Biological Inc., Beijing, China) and subcloned in pCI-neo expression vector using standard procedures (Promega, Madison, WI) and the resulting pCI-neo FGFR2c construct was verified by sequencing. Jun 24, 2019 · signal sequence and cloned into pcDNA4/TO vector (Invitrogen). The constructs encoding HA-tagged Sec31A were generated by cloning different isoforms of Sec31A, followed by the 2xHA tag in pCI-neo vector (Promega). HA-Climp63 was PCR-amplified from mouse cDNA using primers containing an N-terminal HA tag and inserted into pcDNA5/TO To evaluate whether overexpression of Bax, an apoptosis-promoting gene, sensitizes KATOIII gastric cancer cells to apoptosis induced by chemotherapeutic agents, three stable cell lines of KATOIII transfected with Bax (KATOIII-Bax), Bcl-2 (KATOIII-Bcl-2), or control pCI-neo expression vector (KATOIII-pCI-neo) were established. Briefly, MCF7 cells stably expressing empty pCI-neo vector or pCI-neo-Id-1-HA were seeded at 1.5 × 10 3 per well in a 12-well plate on the day before transfection and transiently transfected with a reporter construct (50 ng HRE-Luc or 150 ng VEGF promoter-Luc) and a β-galactosidase expression vector (100 ng) in the maintenance medium with Several strategies of multiple gene expression were tried in Bacteria, Mammalian and Plant cell (LEE J.Het al 2002and Johnston et al 2000 and Ruker.pet al 1997 and Susan et al 2002). vector: PCI-Neo mammalian expression vector (Figure -1) consists of a cytomegalovirus promoter (CMV) to drive the expression of Gene of Interest (GOI).

OBJECTIVE: To study the construction of the PCI-neo mammalian expression vector system containing murine 4-1BBL gene and its stable and effective expression in rat hepatocellular carcinoma cell line CBRH7919. METHODS: The murine full-length 4-1BBL cDNA was obtained and subcloned into the PCI-neo mammalian expression vector.

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The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

Tel: 1-631-626-9181 (USA) 44-208-144-6005 (Europe) Email: pCI-neo Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. OBJECTIVE: To study the construction of the PCI-neo mammalian expression vector system containing murine 4-1BBL gene and its stable and effective expression in rat hepatocellular carcinoma cell line CBRH7919. METHODS: The murine full-length 4-1BBL cDNA was obtained and subcloned into the PCI-neo mammalian expression vector.